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Detection and identification of bacterial DNA in semen
| Authors | Ann A. Kiessling, Ph.D., Bryan M. Desmarais, B.S., Hui-Zhong Yin, M.D., Joseph Loverde, M.S., and Robert C. Eyre, M.D. |
| Title | Detection and identification of bacterial DNA in semen |
| Publication Date | November, 2008 |
| Journal, Volume | Fertility and Sterility, Vol. 90, No. 5, November 2008 |
| Abstract |
Objective: To detect and identify bacteria in semen by sequencing polymerase chain reaction (PCR)–amplified ribosomal RNA gene regions (rDNAs). Design: BacterialrDNAsweredetectedbyPCRamplificationofsemenDNA.Conditionswereadjustedtodetect only abundant organisms, no fewer than 20,000 bacteria/mL of semen. Setting: Clinical andrology laboratory and academic research laboratories. Patient(s): Men undergoing fertility evaluation (n 1⁄4 29) or vasectomy (n 1⁄4 5). MainOutcomeMeaure(s): FrequencyofbacterialrDNA–positivespecimens,relationshipofrDNAstobacteriain GenBank, and correlation with semen cells. Conclusion(s): Abundant bacteria in semen are not commensal, arise from infection in the male genitourinary tract, may influence fertility, and may reflect an inadequate cellular immune response. (Fertil SterilÒ 2008;90: 1744–56. Ó2008 by American Society for Reproductive Medicine.) Key Words: Semen, bacteria, leukocytospermia, semen infection, gram-positive anaerobic cocci, GPAC, sperm, vasectomy, male infertility, male genital tract infection |
| Keywords | bacteria, GPAC, gram-positive anaerobic cocci, leukocytospermia, male genital tract infection, male infertility, Semen, semen infection, sperm, vasectomy |
| Categories | Foundation Newsletters, Science Article Library |
| Link to Article | Detection and identification of bacterial DNA in semen |