Search in library posts
Detection and identification of bacterial DNA in semen
|Authors||Ann A. Kiessling, Ph.D., Bryan M. Desmarais, B.S., Hui-Zhong Yin, M.D., Joseph Loverde, M.S., and Robert C. Eyre, M.D.|
|Title||Detection and identification of bacterial DNA in semen|
|Publication Date||November, 2008|
|Journal, Volume||Fertility and Sterility, Vol. 90, No. 5, November 2008|
Objective: To detect and identify bacteria in semen by sequencing polymerase chain reaction (PCR)–amplified ribosomal RNA gene regions (rDNAs).
Design: BacterialrDNAsweredetectedbyPCRamplificationofsemenDNA.Conditionswereadjustedtodetect only abundant organisms, no fewer than 20,000 bacteria/mL of semen.
Setting: Clinical andrology laboratory and academic research laboratories.
Patient(s): Men undergoing fertility evaluation (n 1⁄4 29) or vasectomy (n 1⁄4 5).
MainOutcomeMeaure(s): FrequencyofbacterialrDNA–positivespecimens,relationshipofrDNAstobacteriain GenBank, and correlation with semen cells.
Conclusion(s): Abundant bacteria in semen are not commensal, arise from infection in the male genitourinary tract, may influence fertility, and may reflect an inadequate cellular immune response. (Fertil SterilÒ 2008;90: 1744–56. Ó2008 by American Society for Reproductive Medicine.)
Key Words: Semen, bacteria, leukocytospermia, semen infection, gram-positive anaerobic cocci, GPAC, sperm, vasectomy, male infertility, male genital tract infection
|Keywords||bacteria, GPAC, gram-positive anaerobic cocci, leukocytospermia, male genital tract infection, male infertility, Semen, semen infection, sperm, vasectomy|
|Categories||Foundation Newsletters, Science Article Library|
|Link to Article||Detection and identification of bacterial DNA in semen|