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February, 2014 | Fertility and Sterility, Volume 101, Issue 2, pages 350-358
Joshua Lupton, Jack Vernamonti, Clinton McCabe, Jacob Noble, Hui Zhong Yin, Robert C. Eyre, Ann A. K  

Cytomegalovirus and human immunodeficiency virus in semen of homosexual men

Objective: To assess the accuracy of serology to predict the presence of cytomegalovirus (CMV) in semen of homosexual men without and with HIV coinfection. Results: Cytomegalovirus was detected by electron microscopy in 3 of 10 specimens examined. Forty-six (89%) of 52 HIV-infected men were seropositive for CMV by combined assay for IgG/IgM; two more (48…

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November, 2008 | Fertility and Sterility, Vol. 90, No. 5, November 2008
Ann A. Kiessling, Ph.D., Bryan M. Desmarais, B.S., Hui-Zhong Yin, M.D., Joseph Loverde, M.S., and Robert C. Eyre, M.D.  

Detection and identification of bacterial DNA in semen

Objective: To detect and identify bacteria in semen by sequencing polymerase chain reaction (PCR)–amplified ribosomal RNA gene regions (rDNAs). Design: BacterialrDNAsweredetectedbyPCRamplificationofsemenDNA.Conditionswereadjustedtodetect only abundant organisms, no fewer than 20,000 bacteria/mL of semen. Setting: Clinical andrology laboratory and academic research laboratories. Patient(s): Men undergoing fertility evaluation (n 1⁄4 29) or vasectomy (n 1⁄4 5). MainOutcomeMeaure(s): FrequencyofbacterialrDNA–positivespecimens,relationshipofrDNAstobacteriain GenBank,…

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