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Detection and identification of bacterial DNA in semen

Authors Ann A. Kiessling, Ph.D., Bryan M. Desmarais, B.S., Hui-Zhong Yin, M.D., Joseph Loverde, M.S., and Robert C. Eyre, M.D.
Title Detection and identification of bacterial DNA in semen
Publication Date November, 2008
Journal, Volume Fertility and Sterility, Vol. 90, No. 5, November 2008

Objective: To detect and identify bacteria in semen by sequencing polymerase chain reaction (PCR)–amplified ribosomal RNA gene regions (rDNAs).

Design: BacterialrDNAsweredetectedbyPCRamplificationofsemenDNA.Conditionswereadjustedtodetect only abundant organisms, no fewer than 20,000 bacteria/mL of semen.

Setting: Clinical andrology laboratory and academic research laboratories.

Patient(s): Men undergoing fertility evaluation (n 1⁄4 29) or vasectomy (n 1⁄4 5).

MainOutcomeMeaure(s): FrequencyofbacterialrDNA–positivespecimens,relationshipofrDNAstobacteriain GenBank, and correlation with semen cells.

Conclusion(s): Abundant bacteria in semen are not commensal, arise from infection in the male genitourinary tract, may influence fertility, and may reflect an inadequate cellular immune response. (Fertil SterilÒ 2008;90: 1744–56. Ó2008 by American Society for Reproductive Medicine.)

Key Words: Semen, bacteria, leukocytospermia, semen infection, gram-positive anaerobic cocci, GPAC, sperm, vasectomy, male infertility, male genital tract infection

Keywords bacteria, GPAC, gram-positive anaerobic cocci, leukocytospermia, male genital tract infection, male infertility, Semen, semen infection, sperm, vasectomy
Categories Foundation Newsletters, Science Article Library
Link to Article Detection and identification of bacterial DNA in semen

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