October, 2011 | Nature, VOL 478
Scott Noggle, Ho-Lim Fung, Athurva Gore, Hector Martinez, Kathleen Crumm Satriani, Robert Prosser, K
The exchange of the oocyte’s genome with the genome of a somatic cell, followed by the derivation of pluripotent stem cells, could enable the generation of specific cells affected in degenerative human diseases. Such cells, carrying the patient’s genome, might be useful for cell replacement. Here we report that the development of human oocytes after…
February, 2011 |
Foundation Staff
Foundation scientists are deriving new stem cell lines from fertilized mouse eggs in fully defined, protein-free culture medium, GEM (Gamete Embryo Medium). GEM is a specialized fluid that simulates fluids in the womb. The goal of these experiments is to improve the efficiency of deriving new stem cell lines from testis biopsies. By studying time lapse videos of…
October, 2010 | Bedford Research Foundation Newsletter
Foundation Staff
Could the newly discovered “testis stem cells” be as versatile as embryonic stem cells? Bedford is launching a major research initiative to find out. A major barrier to progress in stem cell therapies is that, similar to organ transplantation, the body rejects cells that are not a good match for the patient. Surprisingly, stem cells…
March, 2010 | Phil. Trans. R. Soc. B 2010 365, 155-163, doi: 10.1098/rstb.2009.0149.
Watt, Fiona M. and Driskell, Ryan R.
In recent years, there has been an explosion of interest in stem cells, not just within the scientific and medical communities but also among politicians, religious groups and ethicists. Here, we summarize the different types of stem cells that have been described: their origins in embryonic and adult tissues and their differentiation potential in vivo…
January, 2010 | J. Assist. Reprod. Genet., DOI 10.1007/s10815-010-9407-6.
Ann A. Kiessling, Ritsa Bletsa, Bryan Desmarais, Christina Mara, Kostas Kallianidis, Dimitris Loutra
Purpose To understand cell cycle controls in the 8-Cell human blastomere. Methods Data from whole human genome (43,377 elements) microarray analyses of RNAs from normal 8-Cell human embryos were compiled with published microarrays of RNAs from human fibroblasts, before and after induced pluripo- tency, and embryonic stem cells. A sub database of 3,803 genes identified…
February, 2009 | J. Assist. Reprod. Genet., (2009) 26:187 – 195 DOI 10.1007/s10815-009-9306-x.
Ann A. Kiessling, Ritsa Bletsa, Bryan Desmarais, Christina Mara, Kostas Kallianidis, Dimitris Loutra
PURPOSE: To understand the molecular pathways that control early human embryo development. METHODS: Improved methods of linear amplification of mRNAs and whole human genome microarray analyses were utilized to characterize gene expression in normal appearing 8-Cell human embryos, in comparison with published micro-arrays of human fibroblasts and pluripotent stem cells. RESULTS: Many genes involved in…
November, 2008 | Fertility and Sterility, Vol. 90, No. 5, November 2008
Ann A. Kiessling, Ph.D., Bryan M. Desmarais, B.S., Hui-Zhong Yin, M.D., Joseph Loverde, M.S., and Robert C. Eyre, M.D.
Objective: To detect and identify bacteria in semen by sequencing polymerase chain reaction (PCR)–amplified ribosomal RNA gene regions (rDNAs). Design: BacterialrDNAsweredetectedbyPCRamplificationofsemenDNA.Conditionswereadjustedtodetect only abundant organisms, no fewer than 20,000 bacteria/mL of semen. Setting: Clinical andrology laboratory and academic research laboratories. Patient(s): Men undergoing fertility evaluation (n 1⁄4 29) or vasectomy (n 1⁄4 5). MainOutcomeMeaure(s): FrequencyofbacterialrDNA–positivespecimens,relationshipofrDNAstobacteriain GenBank,…
April, 2008 | Fertility and Sterility, Vol. 89, No. 4, April 2008.
Ester Polak de Fried, M.D.,a Pablo Ross, M.Sc.,b Gisela Zang, M.Sc.,a Andrea Divita, M.D.,a Kerriann
Objective: To report on the development of human parthenogenetic blastocysts and an in vitro attachment that was generated from noninseminated cryopreserved human oocytes for the first time. Design: Prospective study. Setting: Department of reproductive medicine in a medical institute in Buenos Aires, Argentina. Patient(s): Five healthy fertile donors. Intervention(s): Artificial activation of noninseminated cryopreserved human…
August, 2007 | Fertility & Sterility - ASRM.
Polak de Fried E, Ross P, Zang G, Divita A, Cunniff K, Denaday F, Salamone D, Kiessling A, Cibelli J
Department of Reproductive Medicine, CER Medical Institute, School of Medicine, Buenos Aires University, Buenos Aires, Argentina. OBJECTIVE: To report on the development of human parthenogenetic blastocysts and an in vitro attachment that was generated from noninseminated cryopreserved human oocytes for the first time. DESIGN: Prospective study. SETTING: Department of reproductive medicine in a medical institute…
July, 2004 | Bedford Stem Cell Research Foundation
Bedford Stem Cell Research Foundation
Bedford Research Foundation is rapidly advancing biomedical research. A Massachusetts public charity, it is setting the standard for ethical and scientific progress in human embryonic stem cell research.
