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February, 2004 | Science Express Report, Page 1/ 10.1126/science.1094515.
Hwang WS, Ryu YJ, Park JH, Park ES, Lee EG, Koo JM, Chun HY, Lee BC, Kang SK, Kim SJ, Ahn C, Hwang  

Evidence of a Pluripotent Human Embryonic Stem Cell Line Derived from a Cloned Blastocyst

Somatic cell nuclear transfer (SCNT) technology has recently been used to generate animals with a common genetic composition. In this study, we report the derivation of a pluripotent embryonic stem cell line (SCNT-hES-1) from a cloned human blastocyst. SCNT-hES-1 cells display typical ES cell morphology and cell surface markers and are capable of differentiating into…

December, 2003 | Human Molecular Genetics, 2004, Vol. 13, No. 7: 727–739.
Amander T. Clark1,2,3, Megan S. Bodnar1,3, Mark Fox1,2,3, Ryan T. Rodriquez1,3, Michael J. Abeyta1  

Spontaneous differentiation of germ cells from human embryonic stem cells in vitro

Little is known of molecular requirements for specification of human germ cells. However, it is likely that they are specified through the action of sequentially expressed genes just as in model organisms. We sought to determine whether human embryonic stem (ES) cell lines, like those of mice, might be capable of forming germ cells in…

April, 2003 | AAAS
American Association for the Advancement of Science  

Regulating Human Cloning

A report on the workshop held March, 2003 by the American Association for the Advancement of Science

November, 2001 | Journal of Regenerative Medicine
Cibelli JB, AA Kiessling, K Cunniff, C Richards, RP Lanza, MD West.  

Somatic Cell Nuclear Transfer in Humans: Pronuclear and Early Embryonic Development

Human therapeutic cloning requires the reprogramming of a somatic cell by nuclear transfer to generate autologous totipotent stem cells. We have parthenogenetically activated 22 human eggs and also performed nuclear transfer in 17 metaphase II eggs. Cleavage beyond the eight-cell stage was obtained in the parthenogenetic-activate d eggs, and blastocoele cavities were observed in six….

November, 1999 | Molecular Medicine, 6(2): 88-95
Joseph Itskovitz-Eldor1, Maya Schuldiner2, Dorit Karsenti1, Amir Eden2, Ofra Yanuka2, Michal Amit1  

Differentiation of Human Embryonic Stem Cells into Embryoid Bodies Comprising the Three Embryonic Germ Layers

Background: Embryonic stem (ES) cells are lines of cells that are isolated from blastocysts. The murine ES cells were demonstrated to be true pluripotent cells as they differentiate into all embryonic lineages. Yet, in vitro differentiation of rhesus ES cells was somewhat inconsistent and disorganized. The recent isolation of human ES cells calls for exploring…

October, 1997 | The Lancet, Vol 350
Byrn R, Zhang D, Eyre R, McGowan K, Kiessling A  

HIV-1 in semen: an isolated virus reservoir

The reservoir of HIV-1 in semen is unknown. Semen is comprised of secretions and cells from seminal vesicles, prostate, testis, epididymis, and ejaculatory ducts; leucocytes are present in semen, although their source and their role in the semen viral burden is unclear. Reports that semen viral RNA concentrations do not correlate with those in plasma,…

January, 1981 | The Journal of Experimental Zoology
Kiessling A, Weitlauf HM  

Poly (A)-Oligo (dT) – Stimulated DNA Polymerase Activity in Preimplantation Mouse Embryos

[Article Removed] This report, published 28 years ago by JEZ, a Wiley-Liss journal, has been replaced with this letter from a representative of Wiley-Liss claiming Bedford Research Foundation owes Wiley-Liss royalties for this article. Under current U. S. government guidelines, put in place in 2008, this article would be available to the general public free…

June, 1976 | Biochemical and Biophysical Research Communications
Kiessling A, Goulian M  

A Comparison of the Enzymatic Responses of the DNA Polymerases From Four RNA Tumor Viruses

[Article removed] This report, published 33 years ago by BBRC, an Elsevier journal, has been replaced with a letter from a representative of Elsevier claiming Bedford Research Foundation owes Elsevier royalties for this article. Under current U. S. government guidelines, put in place in 2008, this article would be available to the general public free…

August, 1944 | American Association for the Advancement of Science
Rock J, Menkin MF  

In Vitro Fertilization and Cleavage of Human Ovarian Eggs

First stages in the cleavage of the fertilized human egg have, as far as we know, never been reported, and while in vitro fertilization of tubal eggs of the rabbit has been described, we have found no record of such experiments in higher mammals. A monkey egg fertilized in vivo has been cultured in vitro…